Thermo-resistant enzyme-producing microorganisms isolated from composting

Abstract Organo-mineral fertilizers supplemented with biological additives are an alternative to chemical fertilizers. In this study, thermoresistant microorganisms from composting mass were isolated by two-step procedures. First, samples taken at different time points and temperatures (33 days 52 ºC, 60 63 over 365 26 ºC) pre-incubated 80 oC for 30 minutes. Second, the microbial selection in vitro culture-based methods heat shock 100 2h 4h. Forty-one isolates able grow °C 4h; twenty-seven 2h, two The molecular identification partial sequencing of 16S ribosomal gene using universal primers revealed that thirty-five eight Bacillus species, one Brevibacillus borstelensis, three Streptomyces thermogriseus, fungi (Thermomyces lanuginosus T. dupontii). Data amylase, phytase, cellulase activity assays enzymatic index (EI) showed 38 41 thermo-resistant produce least enzyme. For amylase activity, highest EI value was observed licheniformis (isolate 21C2, EI= 4.11), followed borstelensis 6C2, 3.66), cereus 18C2, 3.52), paralicheniformis 20C2, 3.34). values 2.30) 3C1, 2.15). Concerning cellulose production, B. altitudinis 6C1) most efficient (EI= 6.40), subtilis (isolates 9C1, 16C2, 19C2) 5.66, 5.84, 5.88, respectively, pumilus 27C2, 5.78). selected potentially useful as a additive organo-mineral other biotechnological processes.